Cnidium Monnieri Extract -Osthole
The main active components of Cnidium extract contain terpene, terpene, bornyl isovalarate, isoborneol, osthole, cindimine. , cididadin, isopimpinelline, etc.Conventional 35%, 50% and other low content is yellow-green powder, and the high content is white needle-like crystalline powder. Soluble in alkali solution, methanol, ethanol, chloroform, acetone, ethyl acetate and zeolite ether, etc., insoluble in water and petroleum ether, mp.83 ° C ~ 84 ° C, bp. 145 ° C ~ 150 ° C.Osthole has antispasmodic, hypotensive, antiarrhythmic, immune-enhancing and broad-spectrum antibacterial effects. Warm kidney and impotence, dampness, hurricane, insecticide. For impotence, palace cold, infertility, cold and wet belt, wet nose and low back pain; external treatment of vulvar eczema, vaginal itching, trichomonas vaginitis.
Pharmacological action of Cnidium Monnieri Extract
Cnidium can inhibit the flocculent epidermidis, gypsum-like bacillus, and lenticular bacillus in the test tube. The newcastle virus cultured in chicken embryo can prolong the life of chicken embryos for 6 hours. Cnidium extract (1 : 2) It has a strong killing effect on vaginal trichomoniasis in vitro. However, it has also been reported that with liver extract as a medium, 10% and 20% of Cnidium decoction has no killing effect or effect on vaginal trichomoniasis.
Continuous injection of C. sinensis ethanol extract into mice for 32 days can prolong estrus and shorten estrus, and can cause estrus in ovariectomized mice, and increase ovarian and uterine weight. Prostate, seminal vesicle, lifting The method of increasing the weight of the anal muscle (mouse) proves that the extract of Cnidium has an androgen-like effect. It has no corrosive effect on the vaginal mucosa of rabbits.
The cnidium extract powder was formulated into a suspension of 20%, 25%, 30%, 35%, 40% with physiological saline, and allowed to stand at room temperature for 48 hours, 200 rpm/separation for 10 minutes, and the supernatant was filtered. Store at 4 ° C. Observe the spermicidal effect with the above medicine, it can be seen that the spermicidal effect increases with the concentration of the drug solution, and the minimum effective concentration for inhibiting human sperm in 20 seconds is 30%, per ml of semen and different doses of Cnidium. The extract powder is mixed, and the minimum effective dose for inhibiting sperm activity is 250~300mg. As shown in the experiment, Cnidium may become a vaginal contraceptive.
The water extract of Cnidium has preventive effects on chloroform-induced ventricular fibrillation, calcium chloride-induced rat ventricular fibrillation and aconitine-induced arrhythmia in rats, and has obvious therapeutic effects on the latter. This indicates that the water extract of Cnidium has an antiarrhythmic effect. It can effectively counter the ectopic rhythm of aconitine promoting sodium channel opening and accelerating Na+ influx. It suggests that Cnidium water extract has Na+ and Ca++ influx in myocardial cell membrane. 4. Obvious inhibition, thus exerting antiarrhythmic effects.
Animal experiments showed that the acetone extract of Cnidium monnieri has strong anti-histamine effect. It can obviously antagonize the contraction of intestinal muscle caused by histamine, chronic reaction substance and 2% egg white. Osthol is administered to mice, small Rat passive skin allergic reaction has a strong inhibitory effect. Osteoinjection by osthole can inhibit the degranulation of rat peritoneal mast cells induced by trichosanthin.
Cnidium decoction alcoholic liquid (100%) has a complete block effect on the action potential of isolated sciatic nerve. Its intensity is the same as that of 10% procaine. It can restore normal conduction function after stopping. It has infiltration in guinea pigs. Anesthetic effect, this effect can be enhanced by adrenaline hydrochloride, but the local anesthetic time is shorter than 10% Procaine; it has spinal anesthesia effect on rabbits, but no surface anesthesia effect on rabbit cornea.
The experiment shows that the water-soluble extract of Cnidium has strong anti-mutagenic properties. It has strong anti-mutagenic effect on the carcinogen aflatoxin B1; it can make SCE (in vitro) induced by dynautic and cyclophosphamide. The frequency of cell sister chromosome exchange decreased; it had antagonistic effect on cyclophosphamide-induced chromosomal aberration and micronucleus in mouse bone marrow cells, and the rate of aberration and micronucleus cell decreased gradually with the increase of dose of Cnidium extract.
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